Sample testing method and sample analyzer

A sample testing method, comprising:

• obtaining a sample to be tested;
• providing a reagent, the reagent comprising at least two fluorescent dyes for staining particles in the sample to be tested, wherein an absolute value of a difference between wavelengths corresponding to peaks of emission spectra of the two fluorescent dyes is greater than 30 nanometers and less than 80 nanometers, and an overlap between the emission spectra of the two fluorescent dyes is not greater than 50%;
• mixing the sample to be tested and the reagent for reaction to form a sample solution to be tested;
• flowing the sample solution to be tested flow in a flow cell in a single test, irradiating the particles flowing in the flow cell by using light with a single wavelength such that the particles generate optical signals, and detecting at least fluorescence signals from the optical signals;
• and
• obtaining a particle test result of the sample to be tested based on at least the fluorescence signals.

A sample analyzer, comprising:

• a sampling portion configured to obtain a sample to be tested and transfer the sample to be tested to a reaction portion;
• a reagent supply portion configured to store a reagent and supply the reagent to the reaction portion as required, wherein the reagent comprises at least two fluorescent dyes for staining particles in the sample to be tested, wherein an absolute value of a difference between wavelengths corresponding to peaks of emission spectra of the two fluorescent dyes is greater than 30 nanometers and less than 80 nanometers, and an overlap between the emission spectra of the two fluorescent dyes is not greater than 50%;
• the reaction portion comprising a mixing chamber and being configured to mix the sample to be tested and the reagent for reaction to form a sample solution to be tested;
• an optical detection system, comprising a first light source, a flow cell, and an optical signal detector, wherein the particles in the sample solution to be tested are capable of flowing in the flow cell in a single test, the first light source is configured to irradiate the particles flowing in the flow cell by using light with a single wavelength such that the particles generate optical signals, and the optical signal detector is configured to detect at least fluorescence signals from the optical signals;
• a transfer apparatus configured to transfer the sample solution to be tested that has been treated with the reagent in the reaction portion, to the flow cell of the optical detection system;
• and
• a processor configured to obtain a particle test result of the sample to be tested, based on at least the fluorescence signals.