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AssigneeAbaxis, Inc.
InventorsRajesh K. MEHRA, Kenneth P. ARON, Vincent CHIANG, Sarah Ann UNSER
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The present invention relates to nanostructure-binding partner conjugates, as well as reaction mixtures, analyte detection devices, and methods of making and using the conjugates. In particular, the invention provides a method of detecting a target analyte in a sample comprising mixing the sample with a first detection conjugate and a second detection conjugate in solution, wherein the first and second detection conjugates comprise metallic nanostructures coupled to binding partners that are capable of specifically binding to the target analyte if present in the sample to form a complex between the first detection conjugate, the analyte, and the second detection conjugate, wherein a change in an optical signal upon complex formation indicates the presence of the target analyte in the sample.
A method of detecting a target analyte in a sample comprising:
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A reaction mixture comprising at least one binding partner-nanostructure conjugate and a zwitterionic detergent, wherein the nanostructures comprise a plurality of protrusions and wherein the average diameter of the nanostructures is from about 50 nm to about 120 nm.
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A method for preparing a conjugate comprising a binding partner and a metallic nanostructure suitable for detecting changes in optical signal based on the presence of a target analyte, wherein the metallic nanostructure comprises a plurality of protrusions and wherein the diameter of the metallic nanostructure is from about 50 nm to about 120 nm, the method comprising:
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Dependencies are inferred from the claim text.