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US 2022/0091108 A1Published application
Dry-down processes for dye-conjugated reagents
AssigneeBeckman Coulter, Inc.
InventorsSridhar Ramanathan, Badri Narayanan Natarajan
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Abstract
The method involves drying down dye-conjugated reagents in separate locations in a reaction vessel so that the dyes don't non-specifically interact with each other during drying. This invention thus improves multiplex binding assays by eliminating erroneous results caused by dyes' being non-specifically attached to each other when dried down together.
Claims20
A reaction vessel comprising a body containing:
- a first dry reactant spot disposed on a substrate, the first dry reactant spot comprising a first binding partner conjugated to a first dye;
- and
- a second dry reactant spot disposed on the substrate, the second dry reactant spot comprising a second binding partner conjugated to a second dye;
- wherein the first dry reactant spot and the second dry reactant spot are at least substantially physically separated on the substrate and the reaction vessel is configured to receive a liquid sample, such that the liquid sample contacts the first dry reactant spot and the second dry reactant spot and dissolves the first binding partner and the second binding partner to form a liquid reaction mixture comprising the first binding partner and the second binding partner in the reaction vessel.
1 more element
A method of preparing two or more dry reactant spots, the method comprising:
- drying a first reactant in liquid phase onto a substrate to form a first dry reactant spot, the first reactant including a first binding partner conjugated to a first dye;
- and
- drying a second reactant in liquid phase onto the substrate to form a second dry reactant spot, the second reactant including a second binding partner conjugated to a second dye, wherein the first dry reactant spot and the second dry reactant spot are at least substantially physically separated on the substrate and wherein at least one of the first dye and second dye is a polymer dye.
Dependencies are inferred from the claim text.