Method of producing test strip for colorimetric detection of calcium content in body fluid using ca-ocpc complex

A method of producing a test strip for measuring a calcium concentration in a sample obtained from a living body, comprising:

• (a) immersing paper in a first reagent and drying it;
• and
• (b) immersing the dried paper in Step (a) in a second reagent and drying it,
• wherein the first reagent is prepared by adding and mixing o-cresolphthalein complexone (OPCP), 8-hydroxyquinoline, sodium lauryl sulphate (SDS) and polyvinylpyrrolidone (PVP) in distilled water, and further adding an organic acid, and
• the second reagent is prepared by adding and mixing N-cyclohexyl-3-aminopropanesulfonic acid and N-methyl-D-glucamine in distilled water, and further adding Triton X-100 and triethylamine borate.