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US 2022/0091103 A1Published application
Vitro assay for drug discovery of valvular heart disease
AssigneeUniversity of South Carolina
InventorMohamad AZHAR
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Abstract
Described herein are assays, methods of use, and novel, stable lines of valve interstitial cells to provide a cell culture-based assay that can be used to quickly identify novel and potential drugs that can used for medical treatment of valve disease since potential therapeutic molecules from mouse VIC assays can be tested in genetic and experimental mouse models in preclinical studies.
Claims14
A cell culture-based valve calcification assay comprising:
- at least one valve interstitial cell;
- wherein the at least one valve interstitial cell is derived from at least one Ts(H2-K1-tsA58) mouse;
- wherein at least one compound is exposed to the at least one valve interstitial cell;
- and
- evaluating the at least one valve interstitial cell to determine if the at least one compound inhibited osteogenic differentiation or calcification in the at least one valve interstitial cell.
2 more elements
A method of identifying new compounds for treatment of valve calcification comprising:
- introducing at least one potential treatment compound to an assay;
- wherein the assay contains at least one valve interstitial cell derived from at least one mouse cell;
- wherein the at least one valve interstitial cell is obtained from at least one Ts(H2-K1-tsA58) mouse and exhibits a pathological processes causing aortic valve and mitral valve calcification;
- and
- evaluating the at least one valve interstitial cell to determine if the at least one potential treatment compound inhibited osteogenic differentiation or calcification in the at least one valve interstitial cell.
2 more elements
A method for obtaining a novel, stable line of valve interstitial cells comprising:
- collecting at least one aortic valve leaflet from at least one Ts(H2-K1-tsA58) mouse;
- centrifuging the at least one aortic valve leaflet;
- digesting the at least one aortic valve leaflet to remove valvular endothelial cells;
- pelleting the at least one aortic valve leaflet by centrifugation;
- washing and repelleting the at least one aortic valve leaflet;
- and
- harvesting at least one valve interstitial cell following digestion.
4 more elements
Dependencies are inferred from the claim text.