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AssigneesKatholieke Universiteit Leuven, Rijksuniversiteit Groningen
InventorsGiovanni Maglia, Mikhael Soskine, Annemie Biesemans, Veerle van Meervelt, Bert Poolman, Gea Schuurman-Wolters
Estimating the term…
A method for detecting an analyte in a sample includes the steps of obtaining a nanopore sensor comprising a nanopore and a protein adaptor internalized in the lumen of the nanopore, adding a sample comprising an analyte to the cis side or the trans side of the nanopore, and measuring conductance across the nanopore. A change in conductance after adding the sample indicates the analyte is present in the sample and has bound to the protein adaptor. Nanopore sensors comprise a nanopore and a protein adaptor internalized in the lumen of the nanopore. The protein adaptor is a functional enzyme or ligand-binding protein.
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A method for detecting a ligand in a sample, comprising the steps of:
A nanopore sensor comprising a biological nanopore and a protein adaptor internalized in the lumen of the nanopore, wherein the protein adaptor is a functional enzyme that binds a ligand while the functional enzyme is internalized, and wherein the sensor is configured to allow measurement of a first conductance change across the nanopore which reflects binding of the ligand to the internalized enzyme adaptor, and to allow measurement of additional conductance changes across the nanopore which reflect enzyme kinetics such as association and dissociation of the ligand and the internalized enzyme adaptor.
Dependencies are inferred from the claim text.