US 2022/0091046 A1Published application

Examination method, system, and computer-readable medium

AssigneeOlympus Corporation

InventorTadashi HIRATA

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Abstract

A method for examining a culture solution containing a pH indicator and accommodated in a culture container includes: measuring, as a baseline intensity, an intensity of light that has passed through a solution and the culture container; measuring, as a measurement intensity, an intensity of light that has passed through the culture solution and the culture container; obtaining light source information including first information pertaining to the light source that is provided when measuring the baseline intensity and second information pertaining to the light source that is provided when measuring the measurement intensity; and on the basis of the baseline intensity, the measurement intensity, and the light source information, calculating an absorbance of the pH indicator at at least one wavelength included in emitted light from the light source.

Claims17

Dependent claims

A method for examining a culture solution containing a pH indicator and accommodated in a culture container, the examination method comprising:

measuring, as a baseline intensity, an intensity of light that is a portion of emitted light from a light source and has passed through a solution not containing the pH indicator and the culture container;
measuring, as a measurement intensity, an intensity of light that is a portion of emitted light from the light source and has passed through the culture solution and the culture container;
obtaining light source information including first information pertaining to the light source that is provided when measuring the baseline intensity and second information pertaining to the light source that is provided when measuring the measurement intensity;
and
on the basis of the baseline intensity, the measurement intensity, and the light source information, calculating an absorbance of the pH indicator at at least one wavelength included in emitted light from the light source, wherein
the measuring the baseline intensity includes
measuring, as a first baseline intensity, an intensity of light that has passed through the solution and the culture container and has a first wavelength at which the absorbance of the pH indicator is pH-dependent, and
measuring, as a second baseline intensity, an intensity of light that has passed through the solution and the culture container and has a second wavelength at which the absorbance of the pH indicator is not pH-dependent,
the measuring the measurement intensity includes
measuring, as a first measurement intensity, an intensity of light that has the first wavelength and has passed through the culture solution and the culture container, and
measuring, as a second measurement intensity, an intensity of light that has the second wavelength and has passed through the culture solution and the culture container,
the obtaining the light source information includes
obtaining, as the first information, a ratio that is attained when measuring the baseline intensity between the intensity of the emitted light from the light source that has the first wavelength and the intensity of the emitted light from the light source that has the second wavelength, and
obtaining, as the second information, a ratio that is attained when measuring the measurement intensity between the intensity of the emitted light from the light source that has the first wavelength and the intensity of the emitted light from the light source that has the second wavelength, and
the calculating the absorbance of the pH indicator includes
calculating the absorbance of the pH indicator at the first wavelength on the basis of the first baseline intensity, the second baseline intensity, the first measurement intensity, the second measurement intensity, the first information, and the second information.

13 more elements

A system comprising:

an image capturing apparatus that has a culture container placed thereon and captures an image of a sample cultured within the culture container;
and
a control apparatus that controls the image capturing apparatus, wherein
the image capturing apparatus includes
a light source,
a photodetector for detecting emitted light from the light source,
the control apparatus
measures, as a baseline intensity by using the photodetector, an intensity of light that is a portion of emitted light from the light source and has passed through a solution not containing a pH indicator and the culture container,
measures, as a measurement intensity by using the photodetector, an intensity of light that is a portion of emitted light from the light source and has passed through a culture solution containing the pH indicator and the culture container,
obtains light source information including first information pertaining to the light source that is provided when measuring the baseline intensity and second information pertaining to the light source that is provided when measuring the measurement intensity,
on the basis of the baseline intensity, the measurement intensity, and the light source information, calculates an absorbance of the pH indicator at at least one wavelength included in emitted light from the light source,
measures, as a first baseline intensity by using the photodetector, an intensity of light that has passed through the solution and the culture container and has a first wavelength at which the absorbance of the pH indicator is pH-dependent,
measures, as a second baseline intensity by using the photodetector, an intensity of light that has passed through the solution and the culture container and has a second wavelength at which the absorbance of the pH indicator is not pH-dependent,
measures, as a first measurement intensity by using the photodetector, an intensity of light that has the first wavelength and has passed through the culture solution and the culture container,
measures, as a second measurement intensity by using the photodetector, an intensity of light that has the second wavelength and has passed through the culture solution and the culture container,
obtains, as the first information, a ratio that is attained when measuring the baseline intensity between the intensity of the emitted light from the light source that has the first wavelength and the intensity of the emitted light from the light source that has the second wavelength,
obtains, as the second information, a ratio that is attained when measuring the measurement intensity between the intensity of the emitted light from the light source that has the first wavelength and the intensity of the emitted light from the light source that has the second wavelength, and
calculates the absorbance of the pH indicator at the first wavelength on the basis of the first baseline intensity, the second baseline intensity, the first measurement intensity, the second measurement intensity, the first information, and the second information.

15 more elements

A non-transitory computer-readable medium having stored therein a program for causing a computer to perform a process comprising:

measuring, as a baseline intensity by using a photodetector, an intensity of light that is a portion of emitted light from a light source and has passed through a solution not containing a pH indicator and a culture container;
measuring, as a measurement intensity by using the photodetector, an intensity of light that is a portion of emitted light from the light source and has passed through a culture solution containing the pH indicator and the culture container;
obtaining light source information including first information pertaining to the light source that is provided when measuring the baseline intensity and second information pertaining to the light source that is provided when measuring the measurement intensity;
on the basis of the baseline intensity, the measurement intensity, and the light source information, calculating an absorbance of the pH indicator at at least one wavelength included in emitted light from the light source;
measuring, as a first baseline intensity by using the photodetector, an intensity of light that has passed through the solution and the culture container and has a first wavelength at which the absorbance of the pH indicator is pH-dependent;
measuring, as a second baseline intensity by using the photodetector, an intensity of light that has passed through the solution and the culture container and has a second wavelength at which the absorbance of the pH indicator is not pH-dependent;
measuring, as a first measurement intensity by using the photodetector, an intensity of light that has the first wavelength and has passed through the culture solution and the culture container;
measuring, as a second measurement intensity by using the photodetector, an intensity of light that has the second wavelength and has passed through the culture solution and the culture container;
obtaining, as the first information, a ratio that is attained when measuring the baseline intensity between the intensity of the emitted light from the light source that has the first wavelength and the intensity of the emitted light from the light source that has the second wavelength;
obtaining, as the second information, a ratio that is attained when measuring the measurement intensity between the intensity of the emitted light from the light source that has the first wavelength and the intensity of the emitted light from the light source that has the second wavelength;
and
calculating the absorbance of the pH indicator at the first wavelength on the basis of the first baseline intensity, the second baseline intensity, the first measurement intensity, the second measurement intensity, the first information, and the second information.

9 more elements

Dependencies are inferred from the claim text.