Back to results
US 2022/0090218 A1Published application
Compositions and methods for detecting human papillomavirus
OwnerYang WU, Gang LIU +2inventors
InventorsYang WU, Gang LIU, Ning LU, Yiyou Chen
Life of this patent
Estimating the term…
Abstract
The present disclosure relates to compositions and methods for detecting and/or identifying the genotype of a human papillomavirus (HPV) in a biological sample obtained from a subject in need thereof. Particularly, the present disclosure provides primers, probes and kits for simultaneously detecting multiple HPV genotypes in a single-tube PCR reaction.
Claims118
A combination of primer and probe for detecting and/or identifying a genotype of a human papillomavirus (HPV), wherein the combination of primer and probe comprises one or more groups of primer and probe selected from the groups consisting of:
- (1) a forward primer comprising a polynucleotide sequence having at least 85%, 90%, 95%, or 100% identity to a sequence of SEQ ID NO:
- 1, a reverse primer comprising a polynucleotide sequence having at least 85%, 90%, 95%, or 100% identity to a sequence of SEQ ID NO:
- 2, and a probe comprising a polynucleotide sequence having at least 85%, 90%, 95%, or 100% identity to a sequence of SEQ ID NO:
- 37;
- (2) a forward primer comprising a polynucleotide sequence having at least 85%, 90%, 95%, or 100% identity to a sequence of SEQ ID NO:
- 3, a reverse primer comprising a polynucleotide sequence having at least 85%, 90%, 95%, or 100% identity to a sequence of SEQ ID NO:
- 4, and a probe comprising a polynucleotide sequence having at least 85%, 90%, 95%, or 100% identity to a sequence of SEQ ID NO:
- 38;
- (3) a forward primer comprising a polynucleotide sequence having at least 85%, 90%, 95%, or 100% identity to a sequence of SEQ ID NO:
- 5, a reverse primer comprising a polynucleotide sequence having at least 85%, 90%, 95%, or 100% identity to a sequence of SEQ ID NO:
- 6, and a probe comprising a polynucleotide sequence having at least 85%, 90%, 95%, or 100% identity to a sequence of SEQ ID NO:
- 39;
- (4) a forward primer comprising a polynucleotide sequence having at least 85%, 90%, 95%, or 100% identity to a sequence of SEQ ID NO:
- 7, a reverse primer comprising a polynucleotide sequence having at least 85%, 90%, 95%, or 100% identity to a sequence of SEQ ID NO:
- 8, and a probe comprising a polynucleotide sequence having at least 85%, 90%, 95%, or 100% identity to a sequence of SEQ ID NO:
- 39;
- (5) a forward primer comprising a polynucleotide sequence having at least 85%, 90%, 95%, or 100% identity to a sequence of SEQ ID NO:
- 9, a reverse primer comprising a polynucleotide sequence having at least 85%, 90%, 95%, or 100% identity to a sequence of SEQ ID NO:
- 10, and a probe comprising a polynucleotide sequence having at least 85%, 90%, 95%, or 100% identity to a sequence of SEQ ID NO:
- 39;
- (6) a forward primer comprising a polynucleotide sequence having at least 85%, 90%, 95%, or 100% identity to a sequence of SEQ ID NO:
- 11, a reverse primer comprising a polynucleotide sequence having at least 85%, 90%, 95%, or 100% identity to a sequence of SEQ ID NO:
- 12, and a probe comprising a polynucleotide sequence having at least 85%, 90%, 95%, or 100% identity to a sequence of SEQ ID NO:
- 40;
- (7) a forward primer comprising a polynucleotide sequence having at least 85%, 90%, 95%, or 100% identity to a sequence of SEQ ID NO:
- 13, a reverse primer comprising a polynucleotide sequence having at least 85%, 90%, 95%, or 100% identity to a sequence of SEQ ID NO:
- 14, and a probe comprising a polynucleotide sequence having at least 85%, 90%, 95%, or 100% identity to a sequence of SEQ ID NO:
- 41;
- (8) a forward primer comprising a polynucleotide sequence having at least 85%, 90%, 95%, or 100% identity to a sequence of SEQ ID NO:
- 15, a reverse primer comprising a polynucleotide sequence having at least 85%, 90%, 95%, or 100% identity to a sequence of SEQ ID NO:
- 16, and a probe comprising a polynucleotide sequence having at least 85%, 90%, 95%, or 100% identity to a sequence of SEQ ID NO:
- 42;
- (9) a forward primer comprising a polynucleotide sequence having at least 85%, 90%, 95%, or 100% identity to a sequence of SEQ ID NO:
- 17, a reverse primer comprising a polynucleotide sequence having at least 85%, 90%, 95%, or 100% identity to a sequence of SEQ ID NO:
- 18, and a probe comprising a polynucleotide sequence having at least 85%, 90%, 95%, or 100% identity to a sequence of SEQ ID NO:
- 42;
- (10) a forward primer comprising a polynucleotide sequence having at least 85%, 90%, 95%, or 100% identity to a sequence of SEQ ID NO:
- 19, a reverse primer comprising a polynucleotide sequence having at least 85%, 90%, 95%, or 100% identity to a sequence of SEQ ID NO:
- 20, and a probe comprising a polynucleotide sequence having at least 85%, 90%, 95%, or 100% identity to a sequence of SEQ ID NO:
- 41;
- (11) a forward primer comprising a polynucleotide sequence having at least 85%, 90%, 95%, or 100% identity to a sequence of SEQ ID NO:
- 21, a reverse primer comprising a polynucleotide sequence having at least 85%, 90%, 95%, or 100% identity to a sequence of SEQ ID NO:
- 22, and a probe comprising a polynucleotide sequence having at least 85%, 90%, 95%, or 100% identity to a sequence of SEQ ID NO:
- 39;
- (12) a forward primer comprising a polynucleotide sequence having at least 85%, 90%, 95%, or 100% identity to a sequence of SEQ ID NO:
- 23, a reverse primer comprising a polynucleotide sequence having at least 85%, 90%, 95%, or 100% identity to a sequence of SEQ ID NO:
- 24, and a probe comprising a polynucleotide sequence having at least 85%, 90%, 95%, or 100% identity to a sequence of SEQ ID NO:
- 40;
- (13) a forward primer comprising a polynucleotide sequence having at least 85%, 90%, 95%, or 100% identity to a sequence of SEQ ID NO:
- 25, a reverse primer comprising a polynucleotide sequence having at least 85%, 90%, 95%, or 100% identity to a sequence of SEQ ID NO:
- 26, and a probe comprising a polynucleotide sequence having at least 85%, 90%, 95%, or 100% identity to a sequence of SEQ ID NO:
- 41;
- (14) a forward primer comprising a polynucleotide sequence having at least 85%, 90%, 95%, or 100% identity to a sequence of SEQ ID NO:
- 27, a reverse primer comprising a polynucleotide sequence having at least 85%, 90%, 95%, or 100% identity to a sequence of SEQ ID NO:
- 28, and a probe comprising a polynucleotide sequence having at least 85%, 90%, 95%, or 100% identity to a sequence of SEQ ID NO:
- 40;
- (15) a forward primer comprising a polynucleotide sequence having at least 85%, 90%, 95%, or 100% identity to a sequence of SEQ ID NO:
- 29, a reverse primer comprising a polynucleotide sequence having at least 85%, 90%, 95%, or 100% identity to a sequence of SEQ ID NO:
- 30, and a probe comprising a polynucleotide sequence having at least 85%, 90%, 95%, or 100% identity to a sequence of SEQ ID NO:
- 40;
- (16) a forward primer comprising a polynucleotide sequence having at least 85%, 90%, 95%, or 100% identity to a sequence of SEQ ID NO:
- 33, a reverse primer comprising a polynucleotide sequence having at least 85%, 90%, 95%, or 100% identity to a sequence of SEQ ID NO:
- 34, and a probe comprising a polynucleotide sequence having at least 85%, 90%, 95%, or 100% identity to a sequence of SEQ ID NO:
- 44;
- (17) a forward primer comprising a polynucleotide sequence having at least 85%, 90%, 95%, or 100% identity to a sequence of SEQ ID NO:
- 35, a reverse primer comprising a polynucleotide sequence having at least 85%, 90%, 95%, or 100% identity to a sequence of SEQ ID NO:
- 36, and a probe comprising a polynucleotide sequence having at least 85%, 90%, 95%, or 100% identity to a sequence of SEQ ID NO:
- 45;
- and any combination thereof.
66 more elements
A DNA chip for testing HPV genotypes, comprising one or more polynucleotides selected from SEQ ID NOs:
- 1 to 45.
A kit for the detection and/or recognition of human papillomavirus (HPV) genotypes in biological samples and for the guidance and efficacy evaluation of HPV vaccine.
A primer, comprising an oligonucleotide sequence having at least 85%, 90%, 95%, or 100% identity to any one of SEQ ID NOs:
- 1-36, wherein the primer has less than 100, 90, 80, 70, 60, 50, 40, 30, or 20 nucleotides.
A pair of primers, comprising a forward primer and a reverse primer, each having at least 85%, 90%, 95%, or 100% identity to any one of SEQ ID NOs:
- 1-36 and each having less than 100, 90, 80, 70, 60, 50, 40, 30, or 20 nucleotides, wherein the forward and reverse primers are selected from the groups consisting of:
- SEQ ID NOs:
- 1-2, 3-4, 5-6, 7-8, 9-10, 11-12, 13-14, 15-16, 17-18, 19-20, 21-22, 23-24, 25-26, 27-28, 29-30, 31-32, 33-34, 35-36, and any combination thereof.
A probe, comprising a fluorescent dye and an oligonucleotide, wherein the oligonucleotide comprises a sequence having at least 85%, 90%, 95%, or 100% identity to any one of SEQ ID NOs:
- 37-45, wherein the oligonucleotide has less than 100, 90, 80, 70, 60, 50, 40, 30, or 20 nucleotides.
Dependencies are inferred from the claim text.