Assay for trichomonas vaginalis by amplification and detection of trichomonas vaginalis ap65-1 gene
AssigneeBecton, Dickinson and Company
InventorRay A. Mc Millian
Life of this patent
Estimating the term…
Abstract
A region of the Trichomonas vaginalis AP65-1 gene has been identified which is useful for performing amplification assays to determine specifically whether T. vaginalis is present in the sample being tested. Oligonucleotides useful for performing thermal Strand Displacement Assay (tSDA) reactions on this gene are disclosed. The disclosed oligonucleotides can be used in an assay which is specific for multiple strains of T. vaginalis and which does not show cross reactivity with the genomes of other microorganisms or with human DNA.
Claims7
1-28. (canceled)
A set of a plurality of primers and a probe for the detection of the AP65-1 gene of Trichomonas vaginalis comprising a first oligonucleotide primer comprising SEQ ID NO:
- 15, a second oligonucleotide primer comprising SEQ ID NO. 16 and a probe comprising SEQ ID NO:
- 17 wherein the first and second oligonucleotide primers and the probe all hybridize within the SEQ ID NO:
- 24 portion of the AP65-1 gene and wherein the probe further comprises a detectable label attached to the probe oligonucleotide.
A set of a plurality of primers and a probe for the detection of the AP65-1 gene of Trichomonas vaginalis comprising a first oligonucleotide primer comprising SEQ ID NO:
- 18, a second oligonucleotide primer comprising SEQ ID NO. 19 and a probe comprising SEQ ID NO:
- 20 wherein the first and second oligonucleotide primers and the probe all hybridize within the SEQ ID NO:
- 24 portion of the AP65-1 gene and wherein the probe further comprises a detectable label attached to the probe oligonucleotide.
A kit for the amplification detection of Trichomonas vaginalis comprising:
- a plurality of primers and a probe for the amplification detection of Trichomonas vaginalis that contains an AP65-1 gene (SEQ ID NO:1)
- a) wherein the plurality of primers comprises a first oligonucleotide primer comprising SEQ ID NO. 15 and a second oligonucleotide primer comprising SEQ ID NO. 16 that bind to the highly conserved region identified as SEQ ID NO:
- 24;
- b) at least one probe comprising SEQ ID NO:
- 17 wherein the probe further comprises a detectable label attached to the probe oligonucleotide.
A kit for the amplification detection of Trichomonas vaginalis comprising:
- a plurality of primers and a probe for the amplification detection of Trichomonas vaginalis that contains an AP65-1 gene (SEQ ID NO:1)
- a) wherein the plurality of primers comprises a first oligonucleotide primer comprising SEQ ID NO. 18 and a second oligonucleotide primer comprising SEQ ID NO. 19 that bind to the highly conserved region identified as SEQ ID NO:
- 24;
- b) at least one probe comprising SEQ ID NO:
- 20 wherein the probe further comprises a detectable label attached to the probe oligonucleotide.
Dependencies are inferred from the claim text.