Clinical assessment of cerebral vasospasm risk following aneurysmal subarachnoid hemorrhage
AssigneeUniversity of Kentucky Research Foundation
InventorWang-Xia Wang
Life of this patent
Estimating the term…
Abstract
A treatment method for cerebral vasospasm involves determining amounts of let-7b-5p, miR-9-3p, miR-142-3p, miR-15b-5p, miR-17-5p, miR-19b-3p, miR-20a-5p, miR-24-3p, miR-29a-3p, miR-125b-5p, and miR-142-3p in a biological sample obtained from a subject following aneurysmal subarachnoid hemorrhage (aSAH) to identify the subject as having an elevated risk of cerebral vasospasm, and administering a treatment to the subject when the elevated risk of cerebral vasospasm is identified.
Claims20
A method for analyzing a biological sample collected from a subject following aneurysmal subarachnoid hemorrhage (aSAH), comprising:
- providing the biological sample collected from the subject following aSAH;
- isolating total RNA from the sample;
- conducting reverse-transcription of miRNAs in the RNA sample to obtain cDNA;
- amplifying the cDNA;
- detecting the cDNA and identifying the miRNA from which the cDNA was transcribed;
- and
- determining amounts of at least five miRNAs in the biological sample, selected from the group consisting of:
- let-7b-5p, miR-9-3p, miR-15b-5p, miR-17-5p, miR-19b-3p, miR-20a-5p, miR-24-3p, miR-29a-3p, miR-125b-5p, and miR-142-3p.
A treatment method, comprising:
- determining amounts of at least five miRNAs selected from the group consisting of let-7b-5p, miR-9-3p, miR-142-3p, miR-15b-5p, miR-17-5p, miR-19b-3p, miR-20a-5p, miR-24-3p, miR-29a-3p, miR-125b-5p, and miR-142-3p in a biological sample obtained from a subject following aneurysmal subarachnoid hemorrhage (aSAH) to identify the subject as having an elevated risk of cerebral vasospasm when there are elevated amounts of the miRNAs relative to control, and
- administering a treatment to the subject when the elevated risk of cerebral vasospasm is identified.
A device for use in identifying risk of cerebral vasospasm in a subject, comprising:
- a combination of probes, including a probe specific for each of at least five miRNAs selected from the group consisting of:
- let-7b-5p, miR-9-3p, miR-15b-5p, miR-17-5p, miR-19b-3p, miR-20a-5p, miR-24-3p, miR-29a-3p, miR-125b-5p, and miR-142-3p.
Dependencies are inferred from the claim text.