Attaching nucleotides to a polynucleotide with a polymerase

Currently amended. A method, comprising:

• hybridizing a polynucleotide to a template, wherein
• the template is bound to a substrate and comprises a first template nucleotide 5-prime adjacent to a 5-prime-most nucleotide of a plurality of nucleotides that are complementary to the polynucleotide and a second template nucleotide 5-prime adjacent to the first template nucleotide,
• contacting the first template nucleotide with a polymerase and a charge-tagged nucleotide, wherein the charge-tagged nucleotide is complementary to the first template nucleotide and comprises a charge tag attached to a 5-prime polyphosphate of the charge-tagged nucleotide,
• attaching the charge-tagged nucleotide to the polynucleotide with the polymerase, wherein the attaching comprises detachment of the charge tag from the charge-tagged nucleotide,
• contacting the second template nucleotide with a fluorescently-tagged nucleotide wherein the fluorescently tagged nucleotide is complementary to the second template nucleotide but not to the first template nucleotide, and
• attaching the fluorescently-tagged nucleotide to the polynucleotide with a polymerase.