Method of nanopore sequencing of concatenated nucleic acids
AssigneeOxford Nanopore Technologies PLC
InventorsAndrew John Heron, Daniel Ryan Garalde, James White
Life of this patent
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Abstract
The invention relates to a new method of characterising two or more target polynucleotides using a pore. The method involves sequentially attaching to a first polynucleotide one or more subsequent polynucleotides to form a concatenated polynucleotide.
Claims20
-38. (canceled)
A population of polynucleotide adaptors comprising a first polynucleotide adaptor and a second polynucleotide adaptor, wherein the first polynucleotide adaptor and the second polynucleotide adaptor each comprise a first polynucleotide strand and a second polynucleotide strand, each strand having a 5′ end and a 3′ end, wherein:
- (a) a portion extending to the 3′ end of the first polynucleotide strand is complementary to a portion extending to the 5′ end of the second polynucleotide strand and the complementary portions form a duplex;
- (b) a single stranded portion extending to the 5′ end of the first polynucleotide strand and a single strand portion extending to the 3′ end of the second polynucleotide strand that are not complementary, do not hybridise to one another;
- and wherein (c) the complimentary portion extending to the 5′ end of the second polynucleotide strand of the first polynucleotide adaptor comprises a sequence that is capable, when the duplex is unwound, of hybridising to a sequence comprised in the single stranded portion extending to the 5′ end of the first polynucleotide strand of the second polynucleotide adaptor.
Dependencies are inferred from the claim text.