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US 2022/0090191 A1Published application
Compositions and methods for nucleic acid sequencing
AssigneeSingular Genomics Systems, Inc.
InventorsEli N. GLEZER, Mohammad Vatankhah VARNOSFADERANI, Daan WITTERS, Vahid KARIMKHANI
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Abstract
Provided herein are methods and compositions for improved sequencing techniques using, for example, polymeric particles and/or three-dimensional structures.
Claims25
-14. (canceled)
A composition comprising a plurality of cores surrounded by a shell polymer, wherein:
- (a) each core of the plurality of cores is surrounded by the shell polymer;
- (b) the core is formed by polymerized units of core monomers forming a core polymer;
- (c) a core polynucleotide primer is attached to the core polymer within the core;
- (d) a target nucleic acid is hybridized to the core primer;
- (e) the shell polymer is formed by polymerized units of shell monomers;
- and
- (f) the shell polymer is not attached to a polynucleotide primer.
4 more elements
-28. (canceled)
(canceled)
-37. (canceled)
-112. (canceled)
A particle comprising a core surrounded by a shell polymer, wherein:
- (a) the core is formed by polymerized units of core monomers forming a core polymer;
- (b) two or more different core polynucleotide primers are attached to the core polymer within the core;
- (c) a target nucleic acid is hybridized to at least one core primer;
- (d) the shell polymer is formed by polymerized units of shell monomers;
- and
- (e) the shell polymer is not attached to a polynucleotide primer.
3 more elements
A particle comprising a core polymer, wherein:
- (a) the core is formed by polymerized units of core monomers forming a core polymer;
- (b) a core polynucleotide primer is attached to the core polymer within the core;
- (c) a target nucleic acid is hybridized to the core primer;
- and
- (d) the particle comprises a silica, magnetic, or paramagnetic bead.
2 more elements
Dependencies are inferred from the claim text.