System using contamination index for evaluating false positive due to contamination by positive control template

Abstract

Disclosed herein is a method for determining a false positive by a real-time nucleic acid amplification reaction, including steps of a) preparing a positive control including a positive control gene including a target gene sequence and a contamination-determining gene sequence, b) obtaining a gene from a sample to prepare a group to be tested, followed by adding an internal control gene to the group to be tested, and c) adding probes capable of binding to each of a target gene, a contamination-determining gene and the internal control gene respectively to the positive control and the group to be tested, followed by proceeding a real-time nucleic acid amplification reaction (PCR), and characterized in that fluorescent light is emitted at the same wavelength when the probes capable of binding to each of the contamination-determining gene and the internal control gene are hydrolyzed.

Dependencies are inferred from the claim text.