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US 2022/0090179 A1Published application
Compositions and methods for detecting an abasic site
AssigneeIllumina, Inc.
InventorsJie Mao, Randall Smith
Life of this patent
Estimating the term…
Abstract
A method for detecting an abasic site is provided. The method may include flowing a solution over a substrate having a plurality of oligonucleotides coupled thereto. At least one of the oligonucleotides includes an abasic site. The solution may include a fluorophore coupled to a reactive group. The method may include reacting the reactive group with the abasic site to couple the fluorophore to the abasic site; and detecting the abasic site using fluorescence from the fluorophore.
Claims33
A method for detecting an abasic site, the method comprising:
- flowing a solution over a substrate having a plurality of oligonucleotides coupled thereto,
- at least one of the oligonucleotides comprising an abasic site,
- the solution comprising a fluorophore coupled to a reactive group;
- reacting the reactive group with the abasic site to couple the fluorophore to the abasic site;
- and
- detecting the abasic site using fluorescence from the fluorophore.
3 more elements
A composition comprising:
- a substrate having a plurality of oligonucleotides coupled thereto,
- at least one of the oligonucleotides comprising an abasic site;
- and
- a fluorophore coupled to the abasic site, the abasic site being detectable using fluorescence from the fluorophore.
1 more element
A method, comprising:
- preparing a solution comprising (i) glycosylases, (ii) oligonucleotides, and (iii) fluorophores coupled to reactive groups;
- generating, using the glycosylases, abasic sites in the oligonucleotides in the solution;
- reacting the reactive groups with the abasic sites to couple the fluorophores to the abasic sites;
- measuring activity of the glycosylases using fluorescence from the fluorophores coupled to the abasic sites;
- and
- using the glycosylases in a sequencing-by-synthesis operation.
3 more elements
33-42. (canceled)
Dependencies are inferred from the claim text.