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AssigneesThe Broad Institute, Inc., Massachusetts Institute of Technology, President and Fellows of Harvard College
InventorsOmar Abudayyeh, James Joseph Collins, Jonathan Gootenberg, Feng Zhang, Eric S. Lander
Estimating the term…
The embodiments disclosed herein utilized RNA targeting effectors to provide a robust CRISPR-based diagnostic with attomolar sensitivity. Embodiments disclosed herein can detect broth DNA and RNA with comparable levels of sensitivity and can differentiate targets from non-targets based on single base pair differences. Moreover, the embodiments disclosed herein can be prepared in freeze-dried format for convenient distribution and point-of-care (POC) applications. Such embodiments are useful in multiple scenarios in human health including, for example, viral detection, bacterial strain typing, sensitive genotyping, and detection of disease-associated cell free DNA.
-30. (canceled)
A diagnostic device comprising one or more individual discrete volumes, each individual discrete volume comprising a CRISPR system comprising a Cas13 effector protein and one or more guide RNAs designed to bind to corresponding target molecules and a masking construct.
The device of previous claim, wherein the hand-held spectrophotometer comprises a smartphone.
The device of previous claim, wherein one or more of the homopolymer sensors comprise a polyU sensor.
The device of previous claim, wherein the isothermal reagents are selected from the isothermal amplification may be nucleic-acid sequenced-based amplification (NASBA), recombinase polymerase amplification (RPA), loop-mediated isothermal amplification (LAMP), strand displacement amplification (SDA), helicase-dependent amplification (HDA), or nicking enzyme amplification reaction (NEAR).
The device of the previous claim wherein the wireless device is a cell phone, a personal digital assistant (PDA) or a tablet.
Dependencies are inferred from the claim text.