Detection method for a target nucleic acid and kit
AssigneesCanon Kabushki Kaisha, Canon Medical Systems Corporation
InventorsMie Okano, Yoji Yamamoto, Masato Minami, Tetsuya Yano
Life of this patent
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Abstract
A detection method for a target nucleic acid comprising: a first step of contacting a target nucleic acid with a first guide RNA and a first Cas protein; a second step of contacting the target nucleic acid with a second guide RNA and a second Cas protein; and a third step of detecting a complex comprising the target nucleic acid, the first guide RNA, the first Cas protein, the second guid RNA, and the second Cas protein, wherein, in the complex, the first guide RNA and the first Cas protein are bound to the first base sequence, and the second guide RNA and the second Cas protein are bound to the second base sequence.
Claims20
A detection method for a target nucleic acid comprising:
- a first step of contacting a target nucleic acid with a first guide RNA and a first Cas protein, the first guide RNA recognizing a first base sequence of the target nucleic acid;
- a second step of contacting the target nucleic acid with a second guide RNA and a second Cas protein, the second guide RNA recognizing a second base sequence of the target nucleic acid and the second base sequence being different from the first base sequence;
- and
- a third step of detecting a complex comprising the target nucleic acid, the first guide RNA, the first Cas protein, the second guide RNA, and the second Cas protein, wherein, in the complex, the first guide RNA and the first Cas protein are bound to the first base sequence, and the second guide RNA and the second Cas protein are bound to the second base sequence.
A kit for detecting a target nucleic acid, the kit comprising a first guide RNA, a first Cas protein, a second guide RNA, and a second Cas protein, wherein
- the first guide RNA has a sequence capable of recognizing a first base sequence of the target nucleic acid,
- the second guide RNA has a sequence capable of recognizing a second base sequence of the target nucleic acid, and
- the second base sequence is different from the first base sequence.
Dependencies are inferred from the claim text.