Asymmetric nanopore membrane (anm) filtration for high-efficiency virus enrichment and purification
OwnerCeming Wang, Hsueh-Chia Changinventors
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Abstract
Described herein is a method for high-efficiency virus enrichment and purification using an asymmetric nanopore membrane (ANM) filtration technology. The ANM design prevents viral particle deformation, lysing, and fusion due to the strong external force and thus significant increases the yield while preserving other advantages of size-based ultrafiltration. It also offers a unique feature of being able to flush the contaminating proteins from the viral particles. It offers higher throughput, yield, sample purity, concentration factor, and more precise size fractionation than current approaches.
Claims29
A system for isolating viral particles comprising:
- a first chamber;
- a second chamber;
- a membrane positioned between the first and second chambers, and comprising a first membrane surface facing and at least partially defining the first chamber, a second membrane surface facing and at least partially defining the second chamber and a plurality of asymmetrically shaped nanopores extending between the first and second membrane surfaces, wherein each nanopore includes a first nanopore opening at the first membrane surface having a first diameter, and a second nanopore opening at the second membrane surface having a second diameter that is greater than the first diameter;
- a sample comprising the viral particles positioned within the first chamber;
- and
- a device for inducing fluid flow through the membrane from the first chamber to the second chamber by pressure driven flow, electroosmotic flow, centrifugal force, or a combination thereof.
A system for isolating viral particles comprising:
- a first chamber;
- a second chamber;
- a membrane positioned between the first and second chambers, and comprising a first membrane surface facing and at least partially defining the first chamber, a second membrane surface facing and at least partially defining the second chamber and a plurality of asymmetrically shaped nanopores extending between the first and second membrane surfaces, wherein each nanopore includes a first nanopore opening at the first membrane surface having a first diameter, and a second nanopore opening at the second membrane surface having a second diameter that is greater than the first diameter;
- wherein the first membrane surface comprises one or more baffles;
- a sample comprising the viral particles positioned within the first chamber;
- and
- a device for inducing fluid flow through the membrane from the first chamber to the second chamber by pressure driven flow, electroosmotic flow, centrifugal force, or a combination thereof.
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Dependencies are inferred from the claim text.