Biosynthetic production of udp-rhamnose
AssigneeConagen Inc.
InventorsGuohong Mao, Phillip James Hunt, Michael James Batten, Timothy Joseph Hanley, Oliver Yu
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Abstract
The present disclosure relates to the biosynthesis of UDP-Rhamnose and recombinant polypeptides having enzymatic activity useful in the relevant biosynthetic pathways for producing UDP-Rhamnose. The present invention also provides a method for preparing a steviol glycoside composition comprising at least one rhamnose-containing steviol glycoside.
Claims26
A biosynthetic method of preparing uridine diphosphate-rhamnose (UDP-rhamnose) from uridine diphosphate-glucose (UDP-glucose), the method comprising incubating UDP-glucose with one or more recombinant polypeptides having UDP-rhamnose synthase activity in the presence of NAD+ and a source of NADPH for a sufficient time to produce UDP-rhamnose.
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A biosynthetic method of preparing a steviol glycoside composition comprising at least one rhamnose-containing steviol glycoside, the method comprising:
- (a) incubating a substrate selected from the group consisting of sucrose, uridine diphosphate and uridine diphosphate-glucose, with one or more recombinant polypeptides having UDP-rhamnose synthase activity in the presence of NAD+ and a source of NADPH to produce uridine diphosphate-rhamnose;
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- (b) reacting the uridine diphosphate-rhamnose with a steviol glycoside substrate in the presence of a recombinant polypeptide having rhamnosyltransferase activity, so that a rhamnose moiety is coupled to the steviol glycoside substrate to produce at least one rhamnose-containing steviol glycoside.
A nucleic acid comprising a sequence encoding a polypeptide comprising an amino acid sequence having at least 99% identity to SEQ ID NO:
- 9, SEQ ID NO:
- 11, SEQ ID NO. 13, SEQ ID NO. 83, SEQ ID NO. 85 or SEQ ID NO. 87.
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Dependencies are inferred from the claim text.