Dna backbone editing for dna data storage
AssigneeSeagate Technology LLC
InventorsGemma MENDONSA, Riyan Alex MENDONSA, Tim RAUSCH
Life of this patent
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Abstract
Methods of writing data to a DNA strand by inserting data-encoding oligos or symbols into a DNA backbone. One particular method of synthesizing a DNA strand encoding data includes cleaving a DNA backbone into multiple segments, and pasting a plurality of data-encoding oligo symbols between the multiple segments, with the terminal ends of the segments joining homologous terminal ends of the symbols, resulting in the DNA strand encoding data comprising alternating segments and symbols.
Claims16
A method of synthesizing a DNA strand encoding data, the method comprising:
- cleaving a DNA backbone into at least three segments, each of the segments having terminal ends;
- and
- pasting a plurality of data-encoding symbols having terminal ends between two of the at least three segments, with the terminal ends of the segments joining homologous terminal ends of the symbols, resulting in the DNA strand encoding data comprising alternating segments and symbols.
A method of synthesizing a DNA strand encoding data, the method comprising:
- cleaving a DNA backbone into multiple segments by moving, via voltage, at least one of the DNA backbone and a Cas9 across a hydrophobic fluidic platform and combining on the platform the DNA backbone and the Cas9 resulting in the multiple segments each having terminal ends;
- and
- moving across the platform, via voltage, a plurality of data-encoding symbols having terminal ends on the platform and inserting the symbols between the multiple segments, with the terminal ends of the segments joining homologous terminal ends of the symbols, forming the DNA strand encoding data comprising alternating segments and symbols.
Dependencies are inferred from the claim text.