Chemically modified lignin as reducing agent for enzymatic hydrolysis of lignocellulosic biomass
AssigneeBorregaard AS
InventorsOskar Bengtsson, Martin Lersch, Anne Mari Kløften
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Abstract
The present invention relates to a method for increasing the rate of enzymatic hydrolysis of a polysaccharide substrate, said method comprising at least one step of: enzymatic hydrolysis of said substrate with a mixture of enzymes, said mixture comprising at least one enzyme selected from lytic polysaccharide monooxygenases; in the presence of chemically modified lignin, wherein during at least part of the time of said step of enzymatic hydrolysis, H2O2 is supplied to the reaction mixture comprising said substrate, said mixture of enzymes and said chemically modified lignin, either from an external source or by generation in situ.
Claims17
A method for enzymatic hydrolysis of a polysaccharide substrate, said method comprising at least one step of:
- enzymatic hydrolysis of said substrate with a mixture of enzymes, said mixture of enzymes comprising at least one enzyme selected from lytic polysaccharide monooxygenases,
- wherein said at least one step of enzymatic hydrolysis occurs in the presence of chemically modified lignin, wherein during at least part of the time of said step of enzymatic hydrolysis, H2O2 is added to a reaction mixture comprising said substrate, chemically modified lignin and said mixture of enzymes.
Dependencies are inferred from the claim text.