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US 2022/0090145 A1Published application
RNA-Guided Targeting of Genetic and Epigenomic Regulatory Proteins to Specific Genomic Loci
AssigneeThe General Hospital Corporation
InventorsJ. Keith Joung, Morgan Maeder
Life of this patent
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Abstract
Methods and constructs for RNA-guided targeting of heterologous functional domains such as transcriptional activators to specific genomic loci.
Claims10
1-25. (canceled)
A method of silencing or repressing expression of a target gene in a cell, the method comprising expressing
- (i) a fusion protein comprising catalytically inactive CRISPR associated 9 (dCas9) protein linked to a heterologous functional domain, wherein the heterologous functional domain is a transcriptional silencer or transcriptional repression domain, and
- (ii) one or more guide RNAs directed to the target gene.
Dependencies are inferred from the claim text.