Transposase with enhanced insertion site selection properties
AssigneeProbiogen AG
InventorsSven Krügener, Thomas Rose, Volker Sandig, Karsten Winkler
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Abstract
The present invention relates to a polypeptide comprising a transposase and at least one heterologous chromatin reader element (CRE). Further, the present invention relates to a polynucleotide encoding the polypeptide. Furthermore, the present invention relates to a vector comprising the polynucleotide. In addition, the present invention relates to a kit comprising a transposase and at least one heterologous chromatin reader element (CRE).
Claims39
A polypeptide comprising a transposase or a fragment or a derivative thereof having transposase function and at least one heterologous chromatin reader element (CRE).
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A kit comprising
- (i) a transposable element comprising a cloning site for inserting at least one polynucleotide of interest, and
- (ii) at least one heterologous CRE and a polypeptide comprising a transposase or a fragment or a derivative thereof having transposase function.
A targeting system comprising
- (i) a transposable element comprising at least one polynucleotide of interest,
- (ii) at least one heterologous CRE, optionally associated with the transposable element, and
- (iii) a polypeptide comprising a transposase or a fragment or a derivative thereof having transposase function.
Dependencies are inferred from the claim text.