Design of bacteriophage-based artificial viruses for human genome remodeling
AssigneeThe Catholic University of America
InventorsVenigalla B. RAO, Jingen ZHU
Life of this patent
Estimating the term…
Abstract
Described is an “artificial virus” (AV) programmed with biomolecules that can enter human cells and carry out precise human genome modification. The AVs comprise: at least one viral vector, such as bacteriophage T4; at least one therapeutic molecule, such as DNA, RNA, protein and their complex; and a lipid coating. Also described is a method of human genome modification, using such an AV, and a method of program such an AV.
Claims24
A human genome remodeling artificial virus (AV) comprising:
- at least one viral vector;
- at least one therapeutic molecule;
- and
- a lipid coating,
- wherein at least one of the therapeutic molecules has gene modification or gene silencing activities.
A human genome remodeling artificial virus (AV) comprising:
- a T4 capsid;
- Cas9 protein;
- at least one RNA;
- at least one DNA;
- and
- a lipid coating,
- wherein the DNA is packaged inside the T4 capsid,
- wherein the RNA is selected from the group consisting of mRNA, siRNA and gRNA,
- wherein the lipid coating comprises at least one cationic lipid.
A method of genome modification comprising:
- infecting animal cells with an artificial virus (AV),
- wherein the AV comprises at least one viral vector;
- at least one therapeutic molecule;
- and
- a lipid coating,
- wherein at least one of the therapeutic molecules has gene modification or gene silencing activities.
(canceled)
(canceled)
Dependencies are inferred from the claim text.