US 2022/0090130 A1 · MODIFIED BACULOVIRUS SYSTEM FOR IMPROVED PRODUCTION OF CLOSED-ENDED DNA (ceDNA)

Abstract

The present disclosure relates to a recombinant baculovirus expression vector (rBEV) for the production of closed-ended DNA (ceDNA) in insect cells.

Dependent claims

A recombinant bacmid, comprising:

(i) a variant of a baculovirus gene required for baculovirus replication, wherein the variant gene exhibits reduced expression of its encoded protein;
(ii) a bacterial origin of replication (ori);
and
(iii) at least one integration site for integration of a heterologous DNA sequence comprising a transgene.

1 more element

Dependencies are inferred from the claim text.

Facts

Application

17/409,774Filed 23 Aug 2021

Priority

23 Aug 2020

Published

24 Mar 2022

Term ends

Not estimatedThe source records don’t support an estimate.

C07K 14/005
Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof: from viruses
C12N 15/86
For animal cells: viral vectors
C07K 14/745
From animals; from humans: blood coagulation or fibrinolysis factors
C07K 14/755
Blood coagulation or fibrinolysis factors: factors VIII , e.g. factor VIII C , factor VIII Ag

The present disclosure relates to a recombinant baculovirus expression vector (rBEV) for the production of closed-ended DNA (ceDNA) in insect cells.

Dependent claims

A recombinant bacmid, comprising:

(i) a variant of a baculovirus gene required for baculovirus replication, wherein the variant gene exhibits reduced expression of its encoded protein;
(ii) a bacterial origin of replication (ori);
and
(iii) at least one integration site for integration of a heterologous DNA sequence comprising a transgene.

1 more element

Dependencies are inferred from the claim text.