Transgenic barb
AssigneeGloFish, LLC
InventorsAlan BLAKE, Richard CROCKETT, Aidas NASEVICIUS
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Abstract
The present invention relates to transgenic ornamental barbs, as well as methods of making such fish by in vitro fertilization techniques. Also disclosed are methods of establishing a population of such transgenic barbs and methods of providing them to the ornamental fish industry for the purpose of marketing.
Claims40
A transgenic Barb comprising a chromosomally integrated expression cassette encoding a fluorescent protein, wherein the Barb comprises the “Orange Barb 1 transformation event,” sperm comprising the Orange Barb 1 transformation event having been deposited at the ATCC as deposit no. PTA-126571.
A method of producing a transgenic Barb comprising:
- (a) obtaining a Barb that comprises a chromosomally integrated expression cassette encoding a fluorescent protein, wherein the Barb comprises the “Orange Barb 1 transformation event,” sperm comprising the Orange Barb 1 transformation event having been deposited at the ATCC as deposit no. PTA-126571;
- and
- (b) breeding the obtained Barb with a second Barb to provide a transgenic Barb comprising the Orange Barb 1 transformation event.
A transgenic Barb comprising a chromosomally integrated expression cassette encoding a fluorescent protein, wherein the Barb comprises the “Purple Barb 1 transformation event,” sperm comprising the Purple Barb 1 transformation event having been deposited at the ATCC as deposit no. PTA-126570.
A method of producing a transgenic Barb comprising:
- (a) obtaining a Barb that comprises a chromosomally integrated expression cassette encoding a fluorescent protein, wherein the Barb comprises the “Purple Barb 1 transformation event,” sperm comprising the Purple Barb 1 transformation event having been deposited at the ATCC as deposit no. PTA-126570;
- and
- (b) breeding the obtained Barb with a second Barb to provide a transgenic Barb comprising the Purple Barb 1 transformation event.
Dependencies are inferred from the claim text.