Transgenic mouse expressing common human light chain
AssigneeCompass Therapeutics LLC
InventorMichael March Schmidt
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Abstract
The present disclosure relates to a transgenic non-human mammal (e.g., mouse) that expresses a common light chain, wherein the transgenic non-human mammal comprises a hybrid light chain locus from which a light chain having a human Vκ joined to a mouse Jκ is expressed. Also provided are methods of making a transgenic non-human mammal capable of expressing a common light chain.
Claims33
A transgenic mouse, comprising in its germline a hybrid variable immunoglobulin (Ig) light chain locus comprising a human light chain Ig V kappa (Vκ) gene segment operably linked to a mouse light chain Ig J kappa (Jκ) gene segment, wherein the mouse light chain Ig Jκ segment is selected from the group consisting of Jκ1, Jκ2, Jκ3, Jκ4, and Jκ5.
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A transgenic mouse, comprising in its germline a hybrid variable immunoglobulin (Ig) light chain locus comprising a human light chain Ig V kappa (Vκ) gene segment operably linked to a mouse light chain Ig J kappa (Jκ) gene segment, wherein a hybrid variable Ig light chain transgene is inserted by site-specific integration to replace an endogenous murine Ig Jκ locus, and wherein hybrid variable Ig light chain locus is operatively linked to an endogenous mouse light chain constant region gene segment, wherein the human light chain immunoglobulin Vκ gene segment comprises W1-39 or W3-15, and wherein the mouse light chain Ig Jκ segment is selected from the group consisting of Jκ1, Jκ2, Jκ3, Jκ4, and Jκ5.
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Dependencies are inferred from the claim text.