Isolated polynucleotides and polypeptides associated with plants resistance to pathogenic fungi
AssigneeEvogene Ltd.
InventorsTidhar TURGEMAN, Ada VITERBO FAINZILBER, Eyal EMMANUEL
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Abstract
The present invention relates to polynucleotides and polypeptides associated with increased resistance of plant to pathogenic fungi, particularly to fungi inducing root rot and stalk rot in plants, and use thereof for controlling plant fungal pathogens and for producing transgenic plants having increased resistance to pathogenic fungi.
Claims20
A method for enhancing the resistance of a plant or a part thereof to at least one pathogenic fungus and/or Oomycete, comprising modulating the expression and/or activity of at least one polypeptide at least 80% identical to a polypeptide having an amino acid sequence selected from the group consisting of SEQ ID NOs:586, 571-585, and 587-939 within at least one cell of the plant or part thereof, thereby enhancing the resistance of said plant or part thereof to the at least one pathogenic fungus and/or Oomycete compared to the resistance of a control plant.
A method for producing a population of plants each having an enhanced resistance to at least one pathogenic fungus and/or Oomycete, comprising the steps of:
- a. modulating the expression and/or activity of at least one polypeptide at least 80% identical to a polypeptide having an amino acid sequence selected from the group consisting of SEQ ID NOs:586, 571-585, and 587-939 within at least one cell of each plant of a plant population as to produce a genetically engineered plant population;
- b. inoculating each plant of the genetically engineered plant population with the at least one pathogenic fungus or Oomycete;
- and c. selecting plants showing an enhanced resistance to said at least one pathogenic fungus or Oomycete compared to a control plant or to a pre-determined resistance score value;
- thereby producing a population of genetically engineered plants having enhanced resistance to said at least one pathogenic fungus and/or Oomycete.
A method for selecting a plant having an enhanced resistance to at least one pathogenic fungus and/or Oomycete, comprising the steps of:
- a. providing a plurality of plants each comprising at least one cell with modulated expression and/or activity of a polypeptide at least 80% identical to a polypeptide having an amino acid sequence selected from the group consisting of SEQ ID NOs:586, 571-585, 587-939;
- b. inoculating the plurality of plants with the at least one pathogenic fungus or Oomycete;
- and c. selecting plants showing an enhanced resistance to said at least one pathogenic fungus or Oomycete compared to a control plant or to a pre-determined resistance score value;
- thereby selecting a plant having enhanced resistance to said at least one pathogenic fungus.
A genetically engineered plant having enhanced resistance to at least one fungus and/or Oomycete compared to a non-engineered control plant, the genetically engineered plant comprises at least one cell having modified expression and/or activity of at least one polypeptide at least 80% identical to a polypeptide having an amino acid sequence selected from the group consisting of SEQ ID NOs:586, 571-585, and 587-939 compared to the polypeptide expression and/or activity in the non-engineered control plant.
A genetically engineered plant having enhanced resistance to the at least one fungus and/or Oomycete comprising at least one cell having enhanced expression and/or activity of at least one polypeptide at least 80% identical to a polypeptide having an amino acid sequence selected from the group consisting of SEQ ID NOs:
- 586, 573, 575-578, 585, 589, 592-594, 600, 607, 609-611, 614, 629-632, 635, 641, 642, 645, and 651-654.
A nucleic acid construct comprising a polynucleotide comprising a nucleic acid sequence at least 80% identical to a polynucleotide having a nucleic acids sequence selected from the group consisting of SEQ ID NOs:70, 136, 57, 59-62, 69, 73, 76-78, 84, 91, 93-95, 98, 113-116, 119, 123, 125-128, 135, 138, 141-143, 149, 156, 158-160, 162, 177-180, and 183, wherein the polynucleotide, when expressed in a plant, is capable of enhancing the resistance of the plant to at least one pathogenic fungus and/or Oomycete.
Dependencies are inferred from the claim text.