Powdery mildew resistant cannabis plants
AssigneeBetterseeds Ltd
InventorsTal SHERMAN, Ido MARGALIT, Shira COREM
Life of this patent
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Abstract
A modified Cannabis plant exhibiting enhanced resistance to powdery mildew (PM). The aforementioned modified Cannabis plant comprises a targeted genome modification conferring reduced expression of at least one Cannabis MLO (CsMLO) allele as compared to a Cannabis plant lacking said targeted genome modification. Methods for production of the modified Cannabis plant using genome modification.
Claims25
-98. (canceled)
A modified Cannabis plant exhibiting enhanced resistance to powdery mildew (PM), wherein said plant comprises at least one targeted genome modification conferring reduced expression of at least one Cannabis MLO (CsMLO) allele as compared to a Cannabis plant lacking said targeted genome modification, said at least one targeted genome modification is in at least one CsMLO allele having a genomic nucleotide sequence selected from the group consisting of CsMLO1 having a sequence as set forth in SEQ ID NO:1 or a functional variant thereof, CsMLO2 having a sequence as set forth in SEQ ID NO:4 or a functional variant thereof and CsMLO3 having a sequence as set forth in SEQ ID NO:7 or a functional variant thereof.
A method of determining the presence of a mutant CsMLO1 nucleic acid in a Cannabis plant comprising at least one of:
- a. assaying said Cannabis plant with primers having nucleic acid sequence as set forth in SEQ ID NO:
- 871 and SEQ ID NO:
- 872;
- and
- b. detecting the presence or absence of a deletion of a nucleotide sequence as set forth in SEQ ID NO.:876, SEQ ID NO.:879 or SEQ ID NO.:881.
An isolated nucleotide sequence having at least 75% sequence identity to a nucleic acid sequence selected from (a) a primer or primer pair sequence comprising SEQ ID NO:871 and SEQ ID NO:872, (b) gRNA comprising SEQ ID NO:10-870, (c) mutated CsMLO1 comprising SEQ ID NO:875, SEQ ID NO:877 and SEQ ID NO:880, (d) wild type CsMLO1, CsMLO2, CsMLO3 comprising SEQ ID NO:1, 2, 4, 5, 7, 8 and SEQ ID NO:
- 873, 876, 879 and 881, and/or an isolated amino acid sequence having at least 75% sequence similarity to an amino acid sequence selected from the group consisting of SEQ ID NO:3, SEQ ID NO:6, SEQ ID NO:9, SEQ ID NO:874, SEQ ID NO:878 and SEQ ID NO:882.
Dependencies are inferred from the claim text.