Rna viral rna molecule for gene editing
AssigneeNomad Bioscience GmbH
InventorsHeike Prochaska, Stefano Torti, Anka Thümmler, Patrick Romer, Anatoli Gititch, Yuri Gleba
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Abstract
The invention provides a plus-sense single-stranded RNA viral RNA molecule, comprising a segment encoding a movement protein, a segment encoding a coat protein and a segment that comprises a guide RNA (gRNA), wherein said RNA molecule can be translated, in infected cells, to a polyprotein comprising the movement protein and the coat protein.
Claims19
A plus-sense single-stranded RNA viral RNA molecule, comprising a segment encoding a movement protein, a segment encoding a coat protein and a segment that comprises a guide RNA (gRNA), wherein said RNA molecule can be translated, in infected cells, to a polyprotein comprising the movement protein and the coat protein;
- or a complementary RNA thereof.
An RNA molecule comprising
- (i) optionally a segment encoding a picornaviral P2A protein,
- (ii) a segment encoding a picornaviral movement protein,
- (iii) a segment encoding a picornaviral coat protein, and
- (iv) a segment comprising a guide RNA (gRNA), preferably a single guide RNA (sgRNA);
- or a complementary RNA thereof.
A process of producing a plus-sense single-stranded picornaviral RNA molecule in cells of a plant or plant cells, said RNA molecule comprising a segment encoding a movement protein, a segment encoding a coat protein and a segment that comprises a guide RNA (gRNA), wherein said RNA molecule can be translated, in infected cells, to a polyprotein comprising the movement protein and the coat protein,
- said process comprising providing a DNA molecule encoding the RNA molecule into said cells of said plant or said plant cells.
A plus-sense single-stranded RNA viral RNA molecule, wherein the RNA molecule is an RNA2 of a segmented nepoviral RNA comprising an inserted gRNA sequence;
- or a complementary RNA thereof.
Dependencies are inferred from the claim text.