Method for producing transformed plant and transformation agent

A method of producing a transformed plant, a seed of the plant, or a callus of the plant, the method comprising the following steps:

• (1-1) a step of bringing an Agrobacterium transformed with an expression vector carrying a gene of interest under control of a promoter capable of inducing expression of the gene of interest in a plant body into contact with a target plant body to inoculate the Agrobacterium thereinto;
• (1-2) a step of cutting the plant body;
• and
• (1-3) a step of placing the cut plant body including a site having introduced therein the gene of interest under high-humidity and dark conditions to obtain a plant body having introduced the gene of interest;
• or
• (2-1) a step of bringing an Agrobacterium transformed with an expression vector carrying a gene of interest under control of a promoter capable of inducing expression of the gene of interest in a plant body into contact with a target plant body to inoculate the Agrobacterium thereinto;
• (2-2) a step of cutting the plant body;
• (2-3) a step of placing the cut plant body including a site having introduced therein the gene of interest under high-humidity and dark conditions;
• and
• (2-4) a step of growing the plant body until a seed is ready to be harvested, to thereby obtain a seed having introduced therein the gene of interest;
• or
• (3-1) a step of bringing an Agrobacterium transformed with an expression vector carrying a gene of interest under control of a promoter capable of inducing expression of the gene of interest in a plant body into contact with a target plant body to inoculate the Agrobacterium thereinto;
• (3-2) a step of placing the inoculated plant body under high-humidity and dark conditions;
• (3-3) a step of cutting the plant body;
• (3-4) a step of placing the cut plant body including a site having introduced therein the gene of interest under high-humidity and dark conditions;
• and
• (3-5) a step of producing a callus from the plant body, and
• wherein the Agrobacterium is an Agrobacterium strain MAFF311303 transformed with the expression vector carrying a gene of interest under control of a promoter capable of inducing expression of the gene of interest in a plant body and with a Ti plasmid which is derived from an Agrobacterium strain MAFF301276 and from which a T-DNA region has been removed, an Agrobacterium strain MAFF311303, an Agrobacterium strain GV310 or an Agrobacterium strain MAFF301276.

A method of producing a seed of a transformed plant, comprising the following steps:

• (1) a step of bringing an Agrobacterium transformed with an expression vector carrying a gene of interest under control of a promoter capable of inducing expression of the gene of interest in a plant body into contact with a target plant body to inoculate the Agrobacterium thereinto;
• and
• (2) a step of growing the plant body until a seed is ready to be harvested, to thereby obtain a seed having introduced therein the gene of interest, and
• wherein the Agrobacterium is an Agrobacterium strain MAFF311303 transformed with the expression vector carrying a gene of interest under control of a promoter capable of inducing expression of the gene of interest in a plant body and with a Ti plasmid which is derived from an Agrobacterium strain MAFF301276 and from which a T-DNA region has been removed, an Agrobacterium strain MAFF311303 strain transformed with an expression vector carrying a gene of interest under control of a promoter capable of inducing expression of the gene of interest in a plant body, or an Agrobacterium strain MAFF301276 transformed with an expression vector carrying a gene of interest under control of a promoter capable of inducing expression of the gene of interest in a plant body.