Method for producing compound with modified mother nucleus
AssigneeJapan Biological Informatics Consortium
InventorsKazuo SHINYA, Haruo IKEDA, Mamoru KOMATSU, Junko HASHIMOTO, Ikuko KOZONE, Takuya HASHIMOTO, Kei KUDO
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Abstract
The present invention provides a method for producing a modified compound, including the following steps: (1) a step of cleaving in vitro using a CRISPR/Cas9 system, a target site in a gene cluster involved in the biosynthesis of a compound, (2) a step of connecting using Gibson assembly in vitro the gene cluster cleaved in step (1) and a polynucleotide for modification, and (3) a step of expressing the modified gene cluster obtained in step (2) in a microorganism expression system.
Claims8
A method for producing a modified compound, comprising the following steps:
- (1) a step of cleaving in vitro using CRISPR/Cas9 system, a target site in a gene cluster involved in the biosynthesis of a compound,
- (2) a step of linking in vitro using Gibson assembly, the gene cluster cleaved in step (1) and a polynucleotide for modification, and
- (3) a step of expressing the modified gene cluster obtained in step (2) in a microorganism expression system.
Dependencies are inferred from the claim text.