Integrated system for library construction, affinity binder screening and expression thereof
AssigneeAdagene Inc.
InventorsFangyong DU, Peter Peizhi LUO
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Abstract
A recombinant polynucleotide suitable for use in a display vector is provided. The recombinant polynucleotide includes from 5′ to 3′: a first nucleic acid sequence (or insert) encoding an amino acid sequence to be displayed on a surface; a first pre-selected restriction site; a second nucleic acid sequence encoding a surface peptide capable of being displayed on the surface; and a second pre-selected restriction site. Corresponding display vectors that can be converted into expression vectors in a high-throughput fashion, as well as methods of use thereof, are also provided.
Claims25
A recombinant polynucleotide comprising from 5′ to 3′:
- a first nucleic acid sequence encoding an amino acid sequence to be displayed on a surface;
- a first restriction site selected from the group consisting of XbaI, NcoI, SalI and XhoI sites;
- a second nucleic acid sequence encoding a surface peptide capable of being displayed on said surface;
- and
- a second restriction site selected from the group consisting of XbaI, NcoI, SalI and XhoI sites;
- wherein the first nucleic acid sequence is engineered in-frame with the second nucleic acid sequence;
- wherein the first and second restriction site, when cleaved by corresponding restriction endonuclease thereto, produce compatible sticky ends.
Dependencies are inferred from the claim text.