Method for isolating nucleic acid, nucleic acid isolation kit, and inspection chip

A nucleic acid isolation method, comprising steps of:

• mixing a specimen containing a nucleic acid with an extraction solution to disperse the nucleic acid in the extraction solution;
• bringing the extraction solution containing the nucleic acid into contact with an anionic adsorbent;
• and
• bringing a collection solution into contact with the anionic adsorbent,
• the extraction solution containing a protein denaturant and a basic compound, and having a pH value equal to or less than a pKa value of a conjugate acid of the basic compound, and
• the collection solution having a pH value equal to or more than the pKa value of the conjugate acid of the basic compound.

A nucleic acid isolation kit, comprising:

• an extraction solution;
• a washing solution;
• a collection solution;
• and
• an anionic adsorbent,
• the extraction solution containing a protein denaturant,
• the washing solution containing a basic compound and having a pH value equal to or less than a pKa value of a conjugate acid of the basic compound, and
• the collection solution having a pH value equal to or more than the pKa value of the conjugate acid of the basic compound.

A nucleic acid isolation kit, comprising:

• an extraction solution;
• a collection solution;
• and
• an anionic adsorbent,
• the extraction solution containing a protein denaturant and a basic compound and having a pH value equal to or less than a pKa value of a conjugate acid of the basic compound, and
• the collection solution having a pH value equal to or more than the pKa value of the conjugate acid of the basic compound.

A test chip comprising:

• an extraction solution holding section in which an extraction solution containing a nucleic acid is held;
• a washing solution holding section in which a washing solution is held;
• a collection solution holding section in which a collection solution is held;
• a collection section containing an anionic adsorbent;
• and
• a nucleic acid analysis section,
• the extraction solution containing a protein denaturant,
• the washing solution containing a basic compound and having a pH value equal to or less than a pKa value of a conjugate acid of the basic compound, and
• the collection solution having a pH value equal to or more than the pKa value of the conjugate acid of the basic compound.

A test chip comprising:

• an extraction solution holding section in which an extraction solution containing a nucleic acid is held;
• a collection solution holding section in which a collection solution is held;
• a collection section containing an anionic adsorbent;
• and
• a nucleic acid analysis section,
• the extraction solution containing a protein denaturant and a basic compound and having a pH value equal to or less than a pKa value of a conjugate acid of the basic compound, and
• the collection solution having a pH value equal to or more than the pKa value of the conjugate acid of the basic compound.