Encapsulated Cells
AssigneeThe Australian National University
InventorsThomas HUBER, Damian VAN RAAD
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Abstract
Provided herein are encapsulated cells that comprise a microbial cell modified to express a cytolytic enzyme and encapsulated with one or more layers of a cationic polymer and one or more layers of an anionic polymer that may be used in cell-free protein synthesis. To this end, methods of utilizing such encapsulated cells in synthesizing a target protein, degrading a contaminant in a contaminated environment and detecting an analyte in a sample are also disclosed herein. Further provided herein, are methods of producing such encapsulated cells.
Claims26
A method of producing encapsulated cells, including the steps of:
- (i) providing a plurality of microbial cells, the microbial cells modified to express a cytolytic enzyme;
- (ii) contacting the microbial cells with a cationic polymer;
- (iii) contacting the microbial cells with an anionic polymer;
- to thereby produce encapsulated cells.
An encapsulated cell comprising a microbial cell modified to express a cytolytic enzyme, the microbial cell encapsulated with one or more layers of a cationic polymer and one or more layers of an anionic polymer.
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Dependencies are inferred from the claim text.