Compositions for increasing polypeptide stability and activity, and related methods
AssigneeSolis Biodyne Oü
InventorsOlev Kahre, Kadri Artma, Tiina Kahre
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Abstract
This disclosure provides peptides, polypeptides, fusion polypeptides, compositions, and methods for enhancing or increasing the stability of a polypeptide (e.g., Taq polymerase). Such peptides, polypeptides, fusion polypeptides, or compositions include polypeptides linked to a peptide tag that enhances the stability of the polypeptide. The peptides, polypeptides, fusion polypeptides, compositions may also enhance the activity, specificity, and/or fidelity of other polypeptides in a reaction mixture. The disclosure also provides methods of using such peptides, polypeptides, fusion polypeptides, compositions.
Claims54
A method of increasing stability of a polypeptide comprising providing a peptide tag that has an amino acid sequence that is at least 70% identical to SEQ ID NO:
- 1.
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23-34. (canceled)
A composition comprising a peptide tag linked to a polypeptide, wherein said peptide tag stabilizes said polypeptide at a temperature between about −10° C. and about 50° C., and wherein said fusion polypeptide does not have the amino acid sequence of SEQ ID NO:
- 2.
A composition comprising:
- (a) a fusion polypeptide comprising a peptide tag linked to a first polypeptide;
- and
- (b) a second polypeptide;
- wherein the peptide tag stabilizes said first polypeptide or said second polypeptide at a temperature between −20° C. and 50° C.
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A fusion polypeptide comprising
- i) a peptide tag has an amino acid sequence that is at least 70% identical to SEQ ID NO:
- 1;
- ii) at least one polypeptide;
- wherein the peptide tag is linked to said at least one polypeptide, and the peptide tag stabilizes the fusion polypeptide at a temperature between −20° C. and 50° C., and wherein the fusion polypeptide does not have an amino acid sequence as shown in SEQ ID NO:
- 2.
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58-68. (canceled)
A polypeptide comprising a peptide tag that has an amino acid sequence that is 50% to 98% identical to SEQ ID NO:
- 1.
A polypeptide comprising a peptide tag that has an amino acid sequence that is at least 70% identical to SEQ ID NO:
- 14.
Dependencies are inferred from the claim text.