Engineered transaminase polypeptides
AssigneeCodexis, Inc.
InventorsJovana Nazor, Christopher Michael Micklitsch, Da Duan, Auric Anthony Sowell-Kantz, Steven Miller, Birgit Kosjek, Inman Farasat, Nicholas M. Marshall
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Abstract
The present invention provides engineered transaminase polypeptides and compositions thereof, as well as polynucleotides encoding the engineered transaminase polypeptides. Methods for producing transaminase enzymes are also provided. In some embodiments, the engineered transaminase polypeptides are optimized to provide improved enzyme properties under minimal aqueous conditions in wet organic solvents.
Claims38
An engineered polypeptide having transaminase activity comprising a polypeptide sequence having at least 85% sequence identity to SEQ ID NO:
- 2, 6, 332, 518 and/or 656, or a functional fragment thereof, wherein the polypeptide sequence of said engineered polypeptide comprises at least one substitution or substitution set and wherein the amino acid positions of said polypeptide sequence are numbered with reference to SEQ ID NO:
- 808, 2, 6, 332, 518 and/or 656.
-8. (canceled)
(canceled)
A polynucleotide sequence encoding at least one engineered polypeptide comprising at least 85% sequence identity to SEQ ID NOS:
- 1, 5, 331, 517, and/or 655.
Dependencies are inferred from the claim text.