Uridine diphosphate-dependent glycosyltransferase enzyme

A uridine diphosphate-dependent glycosyltransferase (UGT) enzyme comprising an amino acid sequence that is at least about 60% identical to the amino acid sequence of SEQ ID NO:

• 5 or SEQ ID NO:
• 6, wherein the UGT enzyme comprises:
• a glycine (G) at the position corresponding to position 54 of SEQ ID NO:
• 5;
• a leucine (L) at the position corresponding to position 111 of SEQ ID NO:
• 5;
• and
• a methionine (M) at the position corresponding to position 183 of SEQ ID NO:
• 5.

A uridine diphosphate-dependent glycosyltransferase (UGT) enzyme comprising an amino acid sequence that has at least about 75% sequence identity to the amino acid sequence of SEQ ID NO:1, and comprising one or more modifications with respect to SEQ ID NO:1 selected from:

• a deletion of one or more amino acids corresponding to E74 to T81,
• a substitution at the position corresponding to position 357, which is optionally glycine,
• a substitution at the position corresponding to position 414, and which is optionally leucine,
• a substitution at the position corresponding to position 29, and which is optionally methionine,
• a substitution at the position corresponding to position 402, which is optionally glutamine,
• a substitution at the position corresponding to position 154, which is optionally cysteine,
• a substitution at the position corresponding to position 194, and which is optionally phenylalanine,
• a substitution at the position corresponding to position 277, and which is optionally isoleucine,
• a substitution at the position corresponding to position 208, and which is optionally glutamine,
• a substitution at the position corresponding to position 140, and which is optionally proline, and
• a substitution at the position corresponding to position 259, and which is optionally glutamic acid.

A uridine diphosphate-dependent glycosyltransferase (UGT) enzyme that is a circular permutant of SrUGT76G1, and optionally having from 1 to 20 or from 1 to 15, or from 1 to 10 amino acid modifications independently selected from amino acid substitutions, deletions, and insertions with respect to the corresponding position of SEQ ID NO:1, with the proviso that the circular permutant one or more modifications with respect to SEQ ID NO:1 selected from:

• a deletion of one or more amino acids corresponding to E74 to T81,
• a substitution at the position corresponding to position 357, which is optionally glycine,
• a substitution at the position corresponding to position 414, and which is optionally leucine,
• a substitution at the position corresponding to position 29, and which is optionally methionine,
• a substitution at the position corresponding to position 402, which is optionally glutamine,
• a substitution at the position corresponding to position 154, which is optionally cysteine,
• a substitution at the position corresponding to position 194, and which is optionally phenylalanine,
• a substitution at the position corresponding to position 277, and which is optionally isoleucine,
• a substitution at the position corresponding to position 208, and which is optionally glutamine,
• a substitution at the position corresponding to position 140, and which is optionally proline, and
• a substitution at the position corresponding to position 259, and which is optionally glutamic acid.