Methods for regulating potency of pluripotent stem cells and applications thereof
AssigneeAcademia Sinica
InventorsJoyce Jean LU, Wei-Ju CHEN
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Abstract
The present invention relates to a method for regulating potency of pluripotent stem cells (PSCs) by modulating expression of podocalyxin-like protein 1 (PODXL) and applications thereof.
Claims28
A method for regulating potency of pluripotent stem cells, comprising exposing the stem cells to an effective amount of a modulator of podocalyxin-like protein 1 (PODXL).
A method for preparing differentiated cells, comprising
- (a) subjecting undifferentiated pluripotent stem cells to a condition suitable for differentiation to produce a cell population that comprises differentiated cells and undifferentiated pluripotent stem cells;
- (b) removing the undifferentiated pluripotent stem cells by exposing the cell population to an effective amount of a podocalyxin-like protein 1 (PODXL) antagonist or an inhibitor of cholesterol synthesis;
- and
- (c) optionally culturing the remaining differentiated cells.
A method for treating teratoma in a subject in need, comprising administering to the subject an effective amount of a podocalyxin-like protein 1 (PODXL) antagonist or an inhibitor of cholesterol synthesis.
A method for upregulating potency of pluripotent stem cells, comprising inducing expression of podocalyxin-like protein 1 (PODXL) in the stem cells.
A method for preparing a chimeric embryo, comprising contacting a fertilized embryo of a non-human host with a human extended pluripotent cell (hEPSC) that comprises a recombinant polynucleotide encoding podocalyxin-like protein 1 (PODXL) and culturing the host embryo in contact with the hEPSC wherein the PODXL is overexpressed to form a chimeric embryo.
A method for generating induced pluripotent stem cells (iPSCs) comprising culturing somatic cells in a condition which allows a proportion of the somatic cells to dedifferentiate into iPSCs, wherein the condition comprises a culture medium which comprises cholesterol.
24-25. (canceled)
A composition for treating somatic cells for generating pluripotent stem cells (iPSCs) therefrom via reprograming comprising cholesterol.
Dependencies are inferred from the claim text.