Generating induced pluripotent stem cells

Abstract

Procedures for generating induced pluripotent stem cells include applying stress to a somatic cell to produce strain that induces the somatic cell to become a pluripotent stem cell without a need for transfection. Generating induced pluripotent stem cells can include forming a droplet that surrounds a somatic cell; and applying mechanical stress to the somatic cell to produce mechanical strain that induces the somatic cell to become a pluripotent stem cell without transfection, where forming the droplet includes thermal inkjet printing. This can also include printing the pluripotent stem cell on a substrate and incubating the pluripotent stem cell. In that case, forming an aggregate composing the pluripotent stem cell can include, after printing and before incubating, positioning the substrate above the pluripotent stem cell whereby gravity exerts a force on the pluripotent stem cell away from the substrate.

Dependencies are inferred from the claim text.