Live-attenuated listeria monocytogenes and methods for using the same
AssigneeZhejiang A & F University
InventorsHouhui SONG, Changyong CHENG
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Abstract
The present invention provides a construction method and application of a live-attenuated Listeria monocytogenes, in which a wild-type strain of Listeria monocytogenes EGD-e is used as construction parental strains, and the residues N478 and V479 of LLO are respectively mutated into plasmid free alanine. The live-attenuated strain of Listeria monocytogenes in the present invention can be used as a live vaccine vector and an immunologic adjuvant.
Claims9
A live-attenuated Listeria monocytogenes, wherein wild-type strain of Listeria monocytogenes EGD-e is used as a construction parental strain, and residues N478 and V479 of the cytolysin lysteriolysin O (LLO) are respectively mutated into alanine without containing plasmids;
- finally obtained live-attenuated strain is named as Lemo-C07, the preservation number of the live-attenuated strain is CGMCC 18647, and the preservation institution is China General Microbiological Culture Collection Center.
Dependencies are inferred from the claim text.