Apparatus and processes for isolating biological material
OwnerJohn OAKEY, John KISIDAY +1inventors
InventorsJohn OAKEY, John KISIDAY, Benjamin NOREN
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Abstract
Embodiments of the present disclosure generally relate to apparatus for isolating biological material, processes for fabricating such apparatus, and processes for using such apparatus. In an embodiment, an apparatus for isolating a biological material is provided. The apparatus includes a fluidic channel disposed over a portion of a substrate. The apparatus further includes a hydrogel structure disposed in the fluidic channel, the hydrogel structure comprising a plurality of wells, wherein each well of the plurality of wells has a diameter from about 1 μm to about 500 μm, the hydrogel structure comprising, in polymerized form, one or more photoreactive monomers.
Claims20
An apparatus for isolating a biological material, comprising:
- a fluidic channel disposed over a portion of a substrate;
- and
- a hydrogel structure disposed in the fluidic channel, the hydrogel structure comprising a plurality of wells, wherein each well of the plurality of wells has a diameter from about 1 μm to about 500 μm, the hydrogel structure comprising, in polymerized form, one or more photoreactive monomers.
A process for forming an apparatus for isolating a biological material, comprising:
- introducing a reaction mixture to a first microfluidic channel, the reaction mixture comprising one or more photoreactive monomers and a photoinitiator;
- and
- polymerizing the reaction mixture using lithography, under polymerization conditions, to form a patterned hydrogel structure comprising a plurality of wells, the plurality of wells configured to isolate a cell, tissue, or other biological material.
A process for isolating a biological material, comprising:
- introducing a sample comprising a biological material to a hydrogel structure, wherein the hydrogel structure:
- comprises, in polymerized form, one or more photoreactive monomers;
- and
- has a plurality of wells, one or more wells of the plurality of wells having a diameter from about 1 μm to about 500 μm, the one or more wells of the plurality of wells is configured to retain the biological material;
- and
- introducing a media to the hydrogel structure to remove a portion of the sample from the hydrogel structure.
Dependencies are inferred from the claim text.