Gh9 endoglucanase variants and polynucleotides encoding same
AssigneeNovozymes A/S
InventorsJens Erik Nielsen, Lars Anderson, Allan Svendsen, Rune Nygaard Monrad, Lars Giger, Vasudeva Prahlada Rao, Sohel Dalal, Santhosh Vasu Mepadam, Marie Thrysoe Kruse, Kasper D. Rand, Pernille F. Jensen
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Abstract
The present invention relates to endoglucanase variants and methods for obtaining endoglucanase variants. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.
Claims15
An endoglucanase variant, comprising an alteration at one or more positions in a chelator-induced instability region selected from the group consisting of:
- i) region 1 corresponding to amino acids 95 to 105 of SEQ ID NO:
- 2,
- ii) region 2 corresponding to amino acids 115 to 138 of SEQ ID NO:
- 2,
- iii) region 3 corresponding to amino acids 210 to 251 of SEQ ID NO:
- 2,
- iv) region 4 corresponding to amino acids 267 to 301 of SEQ ID NO:
- 2,
- v) region 5 corresponding to amino acids 339 to 361 of SEQ ID NO:
- 2,
- vi) region 6 corresponding to amino acids 547 to 595 of SEQ ID NO:
- 2,
- vii) region 7 corresponding to amino acids 612 to 660 of SEQ ID NO:
- 2, and
- viii) region 8 corresponding to amino acids 806 to 828 of SEQ ID NO:
- 2,
- wherein the variant has at least 60% and less than 100% sequence identity to SEQ ID NO:
- 2.
(canceled)
10-14. (canceled)
16-22. (canceled)
Dependencies are inferred from the claim text.