Removal of target cells by circulating virus-specific cytotoxic t-cells using mhc class i comprising complexes

A method for the recombinant production of a complex comprising i) a fusion polypeptide of β2-microglobulin and the extracellular domains α1, α2, and α3 of a class I MHC molecule, ii) a pair of disulfide-linked polypeptide chains derived from an antibody hinge region, and iii) at least one pair of an antibody light chain variable domain and an antibody heavy chain variable domain in a eukaryotic cell, comprising the following steps:

• cultivating a eukaryotic cell comprising one or more nucleic acids encoding the complex, and
• recovering the complex from the cell or the cultivation medium,
• wherein the complex comprises exactly one fusion polypeptide of β2-microglobulin and the extracellular domains α1, α2, and α3 of a class I MHC molecule.