Bispecific antibodies with cleavable c-terminal charge-paired tags

(canceled)

(canceled)

The multi-specific antibody of claim 50, wherein the CH3 domain and the negatively charged domain of the first heavy chain polypeptide are positioned relative to each other in an N-terminal to C-terminal direction and the CH3 domain and the positively charged domain of the second heavy chain polypeptide are positioned relative to each other in an N-terminal to C-terminal direction.

The multi-specific antibody of claim 50, wherein the negatively charged domain comprises at least five consecutive negatively charged amino acid residues and the positively charged domain comprises at least five consecutive positively charged amino acid residues.

The multi-specific antibody of claim 50, wherein the negatively charged amino acid residues are aspartic acid residues and the positively charged amino acid residues are selected from the group consisting of lysine, arginine, and histidine residues.

The multi-specific antibody of claim 50, wherein the negatively charged amino acid residues are aspartic acid residues and the positively charged amino acid residues are lysine residues.

The multi-specific antibody of claim 50, wherein the negatively charged amino acid residues are glutamic acid residues and the positively charged amino acid residues are selected from the group consisting of lysine, arginine, and histidine residues.

The multi-specific antibody of claim 50, wherein the first heavy chain polypeptide further comprises a linker sequence covalently attached to the C-terminus of the CH3 domain and the linker is attached to the N-terminus of the negatively charged domain;

• and
• wherein the second heavy chain polypeptide further comprises a linker sequence covalently attached to the C-terminus of the CH3 domain and the linker is attached to the N-terminus of the positively charged domain.

(canceled)

(canceled)

A multi-specific antibody comprising a first heavy chain polypeptide, a first light chain polypeptide, a second heavy chain polypeptide, and a second light chain polypeptide, wherein

• the first heavy chain polypeptide comprises a CH3 domain and a negatively charged domain comprising at least four consecutive negatively charged amino acid residues and the first heavy chain polypeptide and the first light chain polypeptide bind to a first antigen;
• and
• the second heavy chain polypeptide comprises a CH3 domain and a positively charged domain comprising at least four consecutive positively charged amino acid residues and the second heavy chain polypeptide and the second light chain polypeptide bind to a second antigen.