Protein proximity assay in formalin fixed paraffin embedded tissue using caged haptens

A compound having Formula (IE) or (IF):

• wherein
• “Hapten” is one of a pyrazole, a nitropyrazole, a benzofuran, an oxazole, an oxazole sulfonamide, a thiazole, a thiazole sulfonamide, and derivatives or analogs thereof;
• Y is selected from a carbonyl-reactive group, an amine-reactive group, or a thiol-reactive group;
• A is a group comprising a branched or unbranched, substituted or unsubstituted, saturated or unsaturated aliphatic group having between 1 and 15 carbon atoms, and optionally having one or more heteroatoms selected from the group consisting of O, N, or S;
• X is a bond, or a group comprising a branched or unbranched, substituted or unsubstituted, saturated or unsaturated aliphatic group having between 1 and 30 carbon atoms, and optionally having one or more heteroatoms selected from the group consisting of O, N, or S;
• W is a 6-membered substituted or unsubstituted aromatic group;
• each R1 is independently selected from H, F, Cl, Br, I, —O-methyl, —O— ethyl, —O-n-propyl, —O-iso-propyl;
• —O-n-butyl, —O-sec-butyl, or —O-iso-butyl;
• a —S-alkyl group having between 1 and 4 carbon atoms;
• a —N(H)-alkyl group having between 1 and 4 carbon atoms;
• V is a bond, a substituted or unsubstituted alkyl group having between 1 and 4 carbon atoms, or a substituted or unsubstituted —O-alkyl group;
• R3 comprises a phosphate group, an ester group, a sulfate group, a glycoside group, an amide group, a urea group, and a nitro group;
• q is 0 or 1;
• s is 0 or an integer ranging from 1 to 4;
• and
• t is 0 or 1.

A compound having Formula (IE) or (IF):

• wherein
• “Hapten” is a pyrazole or a derivative or analog thereof;
• Y is selected from a carbonyl-reactive group, an amine-reactive group, or a thiol-reactive group;
• A is a group comprising a branched or unbranched, unsubstituted aliphatic group having between 1 and 4 carbon atoms;
• X is an unsubstituted, saturated or unsaturated aliphatic group having between 1 and 8 carbon atoms, and optionally having one or more heteroatoms selected from the group consisting of O, N, or S;
• W is a 6-membered substituted aromatic group;
• each R1 is independently selected from H, F, Cl, Br, I, —O-methyl, —O— ethyl, —O-n-propyl, —O-iso-propyl;
• —O-n-butyl, —O-sec-butyl, or —O-iso-butyl;
• V is a substituted or unsubstituted —O-alkyl group;
• R3 comprises a phosphate group, an ester group, a sulfate group, a glycoside group, an amide group, a urea group, and a nitro group;
• q is 0 or 1;
• s is an integer ranging from 1 to 4;
• and
• t is.