Amplification/detection kit, photothermal pcr amplification method and microorganism detection method using the same
AssigneeGwangju Institute of Science and Technology
InventorsMin Gon KIM, Bo Bin LEE
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Abstract
Disclosed is an amplification/detection kit comprising: a sample pad on which a PCR reaction solution is placed; a transfer pad positioned in contact with the sample pad; a reaction pad into which the PCR reaction solution flowing from the sample pad through the transfer pad flows and which includes a gold thin film deposited on a first surface; and a sealing tape configured to cover and seal a first surface of the transfer pad and a first surface of the reaction pad.
Claims10
An amplification/detection kit comprising:
- a sample pad on which a PCR reaction solution is placed;
- a transfer pad positioned in contact with the sample pad;
- a reaction pad into which the PCR reaction solution flowing from the sample pad through the transfer pad flows and which includes a gold thin film deposited on a first surface;
- and
- a sealing tape configured to cover and seal a first surface of the transfer pad and a first surface of the reaction pad.
A photothermal PCR amplification method using an amplification/detection kit including a gold thin film formed by depositing gold on a pad with an electron-beam, comprising the steps:
- irradiating a laser having a predetermined intensity and a predetermined wavelength to the gold thin film for a first period;
- stopping the laser irradiation to the gold thin film for a second period subsequent to the first period;
- and
- repeating the irradiating of the laser and the stopping of the laser irradiation at a predetermined number of times.
A microorganism detection method using an amplification/detection kit including a gold thin film formed by depositing gold on a pad with an electron-beam, comprising the steps:
- irradiating a laser having a predetermined intensity and a predetermined wavelength to the gold thin film for a first period;
- stopping the laser irradiation to the gold thin film for a second period subsequent to the first period;
- repeating the steps of irradiating the laser and stopping the laser irradiation at a predetermined number of times;
- and
- measuring light emitted from the amplification/detection kit after the repeating is terminated.
Dependencies are inferred from the claim text.