Heterologous Prime Boost Vaccine
AssigneeBoehringer Ingelheim International GmbH
InventorsGuido WOLLMANN, Krishna DAS, Madiha DEROUAZI, Elodie BELNOUE, Knut ELBERS
Life of this patent
Estimating the term…
Abstract
The present invention pertains to the provision of a vaccine comprising a first component (K) and a second component (V), wherein the first component (K) comprises a complex in which a cell penetrating peptide, an antigenic domain and a TLR agonist are functionally linked and the second component (V) comprises an oncolytic recombinant vesicular stomatitis virus expressing an antigenic domain. The invention further pertains to the use of the inventive vaccine in the treatment of cancer. The invention also provides a recombinant vesicular stomatitis virus expressing an antigenic domain and its use in cancer vaccines.
Claims127
A vaccine comprising a first component (K) and a second component (V), wherein the first component (K) comprises a complex, said complex consisting of or comprising:
- (i) a cell penetrating peptide;
- (ii) an antigenic domain, comprising at least one antigen or antigenic epitope;
- and
- (iii) at least one TLR peptide agonist,
- wherein the components i)-iii) are covalently linked, and
- wherein the second component (V) comprises an oncolytic rhabdovirus.
The vaccine according to any one of the preceding claims, wherein the oncolytic rhabdovirus of the second component (V) is a recombinant rhabdovirus.
A vesicular stomatitis virus, wherein the RNA genome of the vesicular stomatitis virus comprises or consists of an RNA sequence identical or at least 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, or 99% identical to SEQ ID NO:
- 80.
A polypeptide comprising or consisting of an amino acid sequence of SEQ ID NO:
- 60 for use in an immunization regimen in combination with a vesicular stomatitis virus, wherein the RNA genome of the vesicular stomatitis virus comprises or consists of an RNA sequence identical or at least 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, or 99% identical to SEQ ID NO:
- 80.
A vesicular stomatitis virus, wherein the RNA genome of the vesicular stomatitis virus comprises or consists of an RNA sequence identical or at least 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 840%, 850%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, or 99% identical to SEQ ID NO:
- 80 for use in an immunization regimen in combination with a polypeptide comprising or consisting of an amino acid sequence of SEQ ID NO:
- 60.
A kit of parts comprising a polypeptide and a vesicular stomatitis virus, wherein the polypeptide comprises or consists of an amino acid sequence of SEQ ID NO:
- 60, and wherein the RNA genome of the vesicular stomatitis virus comprises or consists of an RNA sequence identical or at least 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, or 99% identical to SEQ ID NO:
- 80.
Dependencies are inferred from the claim text.