Compositions and methods for increasing the viability of freeze-dried probiotics
AssigneesThe Broad Institute, Inc., Massachusetts Institute of Technology
InventorsDaniel S. Collins, Jonathan M. Stokes, Luis R. Soenksen, James J. Collins
Life of this patent
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Abstract
The present disclosure relates to compositions, methods, and kits for increasing the viability of bacteria that have been subjected to freeze-drying/lyophilization. In particular, the disclosure relates to compositions and methods for increasing the viability of living medicines (e.g., probiotics) during lyophilization, oral rehydration, and passage through the gastrointestinal tract
Claims20
A composition comprising a probiotic bacteria population and one or more tardigrade-specific intrinsically disordered proteins (TSIDPs)
A composition selected from the group consisting of:
- A preparation for oral delivery to a subject, the preparation comprising one or more recombinant probiotic bacteria that express one or more tardigrade-specific intrinsically disordered proteins (TSIDPs);
- A preparation for oral delivery possessing freeze-dried recombinant probiotic bacteria;
- A freeze-dried preparation for oral delivery comprising one or more isolated tardigrade-specific intrinsically disordered protein (TSIDPs) and one or more isolated populations of probiotic bacteria;
- A pharmaceutical composition comprising an isolated tardigrade-specific intrinsically disordered protein (TSIDP), an isolated population of probiotic bacteria, and a pharmaceutically acceptable carrier;
- A pharmaceutical composition comprising recombinant probiotic bacteria that express a tardigrade-specific intrinsically disordered protein (TSIDP), and a pharmaceutically acceptable carrier;
- A composition comprising a probiotic bacteria population and one or more natural or synthetic intrinsically disordered proteins (IDPs) that possesses cryoprotective or vitrification properties;
- A preparation for oral delivery to a subject, the preparation comprising one or more recombinant probiotic bacteria that express one or more intrinsically disordered proteins (IDPs);
- A preparation for oral delivery comprising freeze-dried recombinant probiotic bacteria;
- A freeze-dried preparation for oral delivery comprising one or more isolated intrinsically disordered protein (IDPs) and one or more isolated populations of probiotic bacteria;
- A pharmaceutical composition comprising an isolated intrinsically disordered protein (IDP), an isolated population of probiotic bacteria, and a pharmaceutically acceptable carrier;
- and
- A pharmaceutical composition comprising recombinant probiotic bacteria that express an intrinsically disordered protein (IDP), and a pharmaceutically acceptable carrier.
(canceled)
A method selected from the group consisting of:
- A method for treating or preventing a probiotic-treatable disease or disorder in a subject having or at risk of developing the probiotic-treatable disease or disorder, the method comprising administering to the subject:
- (a) a pharmaceutical composition comprising an isolated TSIDP, an isolated population of probiotic bacteria, and a pharmaceutically acceptable carrier, or
- (b) a pharmaceutical composition comprising recombinant probiotic bacteria that express a TSIDP, and a pharmaceutically acceptable carrier;
- and
- A method for treating or preventing a probiotic-treatable disease or disorder in a subject having or at risk of developing the probiotic-treatable disease or disorder, the method comprising administering to the subject:
- (a) a pharmaceutical composition comprising an isolated IDP, an isolated population of probiotic bacteria, and a pharmaceutically acceptable carrier, or
- (b) a pharmaceutical composition comprising recombinant probiotic bacteria that express an IDP, and a pharmaceutically acceptable carrier.
20-38. (canceled)
Dependencies are inferred from the claim text.