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US 2022/0088080 A1Published application
Restimulation of cryopreserved tumor infiltrating lymphocytes
AssigneeIovance Biotherapeutics, Inc.
InventorsIan Frank, Michael T. Lotze
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Abstract
The present disclosure provides methods for re-stimulating TIL populations that lead to improved phenotype and increased metabolic health of the TILs and provides methods of assaying for TIL populations to determine suitability for more efficacious infusion after re-stimulation.
Claims24
A method for expanding tumor infiltrating lymphocytes (TILs) into a therapeutic population of TILs, the method comprising:
- (a) performing a first expansion by (i) thawing cryopreserved dissociated tumor materials comprising a first population of TILs obtained from a tumor that was resected from a subject, and (ii) culturing the first population of TILs in a cell culture medium comprising IL-2 to produce a second population of TILs;
- (b) performing a second expansion by supplementing the cell culture medium of the second population of TILs with additional IL-2, OKT-3, and antigen presenting cells (APCs), to produce a third population of TILs, wherein the third population of TILs is a therapeutic population of TILs, and wherein the second expansion is performed for about 7 to 11 days before being split into more than one container or flask in order to obtain the third population of TILs;
- (c) harvesting the third population of TILs obtained from step (b);
- (d) transferring the harvested third population of TILs from step (c) into an infusion bag;
- and
- (e) cryopreserving the infusion bag comprising the harvested TIL population from step (d) using a cryopreservation process.
3 more elements
Dependencies are inferred from the claim text.